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50 Questions and Answers on Microbiology

  1. What is microbiology?
    • The study of microorganisms.
  2. What are microorganisms?
    • Organisms that are too small to be seen with the naked eye.
  3. Who is considered the father of microbiology?
    • Antonie van Leeuwenhoek.
  4. What is a bacterium?
    • A type of single-celled microorganism that has a prokaryotic cell structure.
  5. What is a virus?
    • A non-living infectious agent composed of genetic material (DNA or RNA) enclosed in a protein coat.
  6. What is a fungus?
    • A type of microorganism that includes yeasts, molds, and mushrooms.
  7. What is a protist?
    • A diverse group of eukaryotic microorganisms that are not fungi, plants, or animals.
  8. What is a parasite?
    • An organism that lives on or in a host organism and derives nutrients from it.
  9. What is a pathogen?
    • An organism that causes disease.
  10. What is aseptic technique?
    • Procedures used to prevent contamination of cultures and samples by unwanted microorganisms.
  11. What is sterilization?
    • The process of killing or removing all microorganisms and their spores from an object or environment.
  12. What is pasteurization?
    • The process of heating food or liquids to a specific temperature for a certain period to kill pathogens and extend shelf life.
  13. What is a culture medium?
    • A solid or liquid nutrient solution used to grow and maintain microorganisms in the laboratory.
  14. What is a colony?
    • A visible mass of microorganisms that originated from a single cell.
  15. What is asepsis?
    • The absence of pathogenic microorganisms.
  16. What is a biofilm?
    • A community of microorganisms that are attached to a surface and enclosed in a matrix of extracellular polymeric substances.
  17. What is a prokaryote?
    • A single-celled organism that lacks a nucleus and membrane-bound organelles.
  18. What is a eukaryote?
    • An organism with cells that contain a nucleus and membrane-bound organelles.
  19. What is the Gram stain used for?
    • To classify bacteria into Gram-positive and Gram-negative based on differences in cell wall structure.
  20. What is antibiotic resistance?
    • The ability of bacteria and other microorganisms to resist the effects of antibiotics.
  21. What is the microbiome?
    • The collection of microorganisms that inhabit a particular environment, such as the human body.
  22. What is bioremediation?
    • The use of microorganisms to break down or neutralize pollutants in the environment.
  23. What is fermentation?
    • The metabolic process by which microorganisms convert sugars into acids, gases, or alcohol in the absence of oxygen.
  24. What is a bacteriophage?
    • A virus that infects and replicates within bacteria.
  25. What is a prion?
    • An infectious agent composed of protein that causes neurodegenerative diseases.
  26. What is a viroid?
    • A plant pathogen composed of a single-stranded RNA molecule that causes disease.
  27. What is a toxin?
    • A poisonous substance produced by microorganisms.
  28. What is a mycosis?
    • A disease caused by a fungus.
  29. What is a protozoan?
    • A single-celled eukaryotic organism, such as amoeba or malaria parasite.
  30. What is zoonosis?
    • An infectious disease that can be transmitted from animals to humans.
  31. What is antigen?
    • A molecule that triggers an immune response by binding to antibodies or T-cell receptors.
  32. What is the Koch’s postulates?
    • A set of criteria used to establish the causative relationship between a microorganism and a disease.
  33. What is quorum sensing?
    • The ability of bacteria to communicate and coordinate gene expression based on cell density.
  34. What is phagocytosis?
    • The process by which cells engulf and digest solid particles, such as bacteria or cellular debris.
  35. What is a plasmid?
    • A small, circular DNA molecule found in bacteria that can replicate independently of the chromosomal DNA.
  36. What is recombinant DNA technology?
    • The process of combining DNA molecules from different sources to create genetically modified organisms or produce specific proteins.
  37. What is a vector in molecular biology?
    • A DNA molecule, such as a plasmid or virus, used to carry foreign genetic material into a host cell.
  38. What is PCR (polymerase chain reaction)?
    • A laboratory technique used to amplify and make copies of a specific DNA sequence.
  39. What is gene expression?
    • The process by which information from a gene is used to synthesize a functional gene product, such as protein or RNA.
  40. What is CRISPR-Cas9?
    • A revolutionary gene-editing tool that allows precise modification of DNA sequences.
  41. What is an endospore?
    • A dormant, tough, and non-reproductive structure formed by certain bacteria as a response to adverse environmental conditions.
  42. What is transformation in bacteria?
    • The process by which bacteria take up and incorporate foreign DNA from the environment into their genome.
  43. What is conjugation in bacteria?
    • The process by which bacteria transfer genetic material through direct cell-to-cell contact using a conjugation pilus.
  44. What is transduction in bacteria?
    • The process by which bacterial DNA is transferred from one bacterium to another by a bacteriophage.
  45. What is the role of nitrogen-fixing bacteria?
    • To convert atmospheric nitrogen into ammonia, making it available to plants in a usable form.
  46. What are extremophiles?
    • Microorganisms that thrive in extreme environmental conditions, such as high temperatures, acidity, or salinity.
  47. What is symbiosis?
    • A close and long-term interaction between two different biological species.
  48. What is a commensal microorganism?
    • A microorganism that benefits from its association with another organism without causing harm.
  49. What is mutualism?
    • A symbiotic relationship in which both organisms benefit from the association.
  50. What is parasitism?
    • A symbiotic relationship in which one organism (parasite) benefits at the expense of another organism (host).

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